Aseptic non touch technique (ANTT®) for intravenous therapy

The purpose of this guideline is to provide guidance about aseptic non touch technique (ANTT) for intravenous therapy at Great Ormond Street Hospital (GOSH).

The Aseptic Non Touch Technique (ANTT®) is the standard intravenous technique used for the accessing of all venous access devices (VADs) regardless of whether they are peripherally or centrally inserted (Rowley et al 2010, Loveday et al 2014) and is the de facto standard aseptic technique in the UK (Rowley and Clare 2011).

Poor standards of aseptic technique are a fundamental cause of healthcare acquired infections (Department of Health 2003). The main focus of ANTT® is to minimise the introduction of micro-organisms, which may occur during preparation, administration and delivery of IV therapy. In order to further reduce the potential for contamination, the technique follows some fundamental rules pertaining to infection control and staff/patient protection such as effective handwashing, the wearing of non-sterile gloves, the principles of key parts/sites and the use of alcohol-based solutions for decontamination with adequate cleaning and natural evaporation of the alcohol. 

Furthermore, standard ANTT® is basic in nature and clearly defined, focusing on the essentials of all IV therapy regardless of intravenous device, administration route or clinical condition (Rowley et al 2010).

Key parts and key sites

The underlying principles of ANTT are the protection of key parts and key sites, hence the concept of ‘key parts and key sites’ was applied to all types of IV therapy with the focus being on the equipment used and the protection of key equipment parts. 

These key parts are the pieces of equipment that come into direct contact with the patient and therefore have the potential to transmit bacteria and/or micro-organisms and are usually parts of equipment that come into direct contact with the infusate. 

Remember: identifying and protecting key parts and key sites is paramount. Refer to table 1.1 for a list of key parts/sites.

Table 1.1 list of key parts and key sites

  • Syringe tip.
  • Needle – both the needle tip and the needle hub.
  • Needleless access device attached on catheter lumen.
  • IV infusion lines – includes several key parts such as fluid bag spikes, all bungs/caps/three-way taps, all infusion ports, the end of the infusion line which connects to the patient.
  • Extension lines, eg Lectrocath® (both the end that attaches to the IV administration set and the point where tubing connects to the patient).
  • The hub of the central venous access device (CVAD)/venous access device (VAD).
  • The tip of the implanted port needle and the hub end.
  • Dressings – the parts of dressings that come into direct contact with skin.
  • Sponge sections of the 2% chlorhexidine/70% alcohol applicators (ie ChloraPrep®).
  • Sterile gauze – the centre of the sterile gauze squares (used for dressing changes).
  • Rubber tops of vials containing medications, etc.
  • Ends of bungs used to protect syringe tips.

This is not an exhaustive list.


Well-fitting gloves are another essential part of ANTT®. They should be neither too small, with the potential to be punctured by wearer's fingernails, nor too large, as they may impede manual dexterity (Royal College of Nursing (RCN) 2010). 

Cleaning IV trays

Correct decontamination of the IV trays is an essential part of the process. After the IVs have been administered, the IV trays are decontaminated as follows:

  • Prior to use, the tray needs to be disinfected with a 70% alcohol wipe (eg Sani-Cloth70) and allowed to dry naturally.

  • After use and prior to leaving the patient's bedspace or room, the outside of the tray must be decontaminated with a sanitising wipe (eg Clinell® universal wipe)

  • The tray must then be washed with hot water and liquid (dishwashing) detergent in an equipment washing sink. Then dried using paper towels (do not leave tray to drip dry). NB Hibiscrub® or any other liquid hand soap are not suitable for washing IV trays.

  • If an equipment washing sink is not available the tray can be cleaned using a sanitising wipe (e.g. Clinell® universal wipe). The tray should be allowed to dry naturally (do not use a paper towel to dry the tray.

  • Store all trays away from sink area to prevent re-contamination with water splashes.

Disinfection of needleless access devices and catheter hubs

A 30 second scrub is currently advocated at GOSH using a clock for timings. Counting is very rarely accurate.

The effect of disinfection duration has a correlation with bacterial load on catheter hubs (Simmons et al 2011). Current recommendations from EPIC 3 (Loveday et al 2014, NICE (2012)) and the Centre for Disease Control (CDC) Guidelines (O’Grady et al 2011) are to use wipes containing 2% chlorhexidine in 70% isopropyl alcohol to clean the hubs of venous access devices and the needleless devices for between 15-30 seconds with friction and allowed to dry prior to use (Simmons et al 2011, Soothill et al 2009). Manufacturers’ guidelines advocate a 30-second scrub. ANTT® advocates the use of a 20-second scrub by placing the hub/needleless device tip in the centre of the wipe and scrubbing firmly before cleaning areas away from the tip. A study in a paediatric population recommended using 2% CHG/70% isopropanol and a 30-second scrub (Soothill et al 2009). Proper disinfection of the IV connector is crucial in preventing bacterial contamination of the intraluminal fluid pathway (Macklin 2010). 

A study by Kaler & Chinn (2007) and Smith et al (2012) showed that prolonged contact with the wipe and the use of friction was effective in disinfecting needleless devices. Individually packaged containing 2% chlorhexidine in 70% isopropyl alcohol (Clinell®, Sani-Cloth® CHG 2%) are easily available and should be used as per recommendations.

If alcohol-based products are not allowed to dry naturally, then the antibacterial properties of the agent will be ineffective, placing the patient at risk of developing an infection (O’Grady et al 2002, Rowley et al 2010, RCN 2010).

A 30-second scrub is currently advocated at GOSH using a clock for timings. Counting is very rarely accurate.

Micro critical aseptic fields

Maintaining the asepsis of all key parts is essential. This can be achieved with caps and covers. Individually packaged sterile caps are used to maintain a micro critical asepsis on syringe tips and any key parts.

Accessing venous access devices

When applying ANTT® to intravenous therapy procedures, the actions required are the same when accessing all venous access devices whether they are peripheral, midlines or central. 

It is essential that all staff involved in accessing VADs are fully trained to undertake the procedure and have been deemed IV competent.

Step-by-step guide for ANTT

  1. Put on a (red) plastic apron (Rationale 1).
  2. Wash hands for 30 seconds as per the Hand Hygiene Clinical Practice Guideline (CPG) using an appropriate hand-washing agent and dry thoroughly using hand towels.
  3. Collect plastic tray. Immediately prior to use, the dry tray should be inspected for cleanliness and wiped with a 70% isopropyl alcohol wipe (eg Sani-Cloth 70®). Disinfect all surfaces of the tray internally and then externally. Once cleaned, allow time to dry naturally. 
  4. Collect all necessary equipment, diluents, heparin, medications, etc. Calculate all medication dosages and any dilutions required. Write all required labels (Rationale 2).
  5. Wash hands for 30 seconds as per Hand Hygiene CPG, or use an alcohol hand rub (WHO 2009), (Rationale 3).
  6. Put on pair of well-fitting non-sterile gloves straight from the box. 
  7. Open equipment by carefully peeling back packaging. Place syringe on the tray. Ensure key parts are uppermost/not in contact with tray (Rationale 4).
  8. Connect all needles to syringes and draw up and prepare all medications. Ensure all key parts remain uncontaminated. If at any time you think you may have contaminated a piece of equipment, dispose of it immediately and use a new piece (Rationale 5).
  9. When all equipment/medication has been prepared, remove all needles from syringes and cover syringe tips with a sterile cap (Rationale 6).
  10. Take tray to the child/young person. 
  11. Locate the VAD to be used. Check the VAD and exit site.
  12. If your gloves have become contaminated between preparation of IVs and going to the patient or from exposing the VAD remove gloves, perform hand hygiene and don a fresh pair of gloves (Rationale 7).
  13. Firmly clean the needleless access device attached to the end of the VAD with a 2% chlorhexidine/70% alcohol wipe (eg Clinell®) for 30 seconds using friction and different parts of the wipe. Allow to dry naturally, visibly checking that it is dry (Rationale 8).
  14. Carefully remove cap from the syringe taking care not to contaminate the syringe tip. Insert syringe(s) into the needleless access device of the VAD and administer medications as prescribed. Remember to keep all syringe tips uppermost and not in contact with the plastic tray. 
  15. When all medications have been administered, replace the child’s VAD to ensure safety (Rationale 9).
  16. Dispose of all used equipment as per the 'Sharps: disposal of used sharps' guideline and Waste disposal policy.
  17. Decontaminate the outside of the plastic tray prior to leaving the patient’s bed space or room, with a sanitising wipe (eg Clinell® universal wipe) and then by washing with liquid detergent and drying straight away, or (if no equipment cleaning sink available) clean the tray using a sanitising wipe (eg Clinell® universal wipe). Store in appropriate area away from the sink to avoid re-contamination.
  18. If not already done so, remove gloves and wash hands thoroughly using an appropriate cleansing solution and dry thoroughly (Rationale 10).

Table 1.2: Tips for maintaining ANTT  

  • Do not drop your equipment into your tray.

  • Ensure other equipment in the tray does not come into contact with the key parts (eg white bungs, blood bottles rolling around tray, extension tubing being placed on top of equipment/key parts). 
  • Remember to use the plastic trays/trolley. Always clean correctly before and after use. 
  • Do not use paper trays for IV preparations. 
  • Change gloves if they may have been contaminated. 
  • Gloves are not a replacement for good hand hygiene; therefore, staff must decontaminate their hands before donning and after removing gloves (Rationale 11).
  • Due to hand contamination that occurs when collecting equipment and touching cupboard handles, etc. the aseptic hand clean must occur after this part of the process has been completed. 
  • Take care when inserting the needle into vials/ampoules not to touch the outer sides. 
  • Take care not to contaminate syringe tips when placing and removing covering bungs.


Rationale 1: Dedicated coloured aprons specifically for IVs send a clear message to other people that IV administration is being undertaken and to help prevent interruptions that may lead to distractions and errors.
Rationale 2: To ensure the procedure is taken efficiently and prevent later contamination of gloves.
Rationale 3: To prevent any cross contamination from collecting equipment.
Rationale 4: To prevent contamination of key parts.
Rationale 5: Using potentially contamination equipment can lead to an increased risk of infection.
Rationale 6: To prevent any risk of airborne contamination or key part contamination.
Rationale 7: To minimise cross contamination.
Rationale 8: To ensure adequate cleaning of the device/bung and enable the product to work effectively.
Rationale 9: Helps to prevent accidental dislodgement or damage to the VAD.
Rationale 10: To prevent cross contamination.
Rationale 11: To prevent cross contamination as the warm damp environment under gloves means that bacteria reproduce easily.


Reference 1:
Department of Health (2003) Winning ways, working together to reduce Healthcare Associated Infection in England. Report from the Chief Medical Officer, The Department of Health

Reference 2:
Kaler, W and Chinn (2007) Successful disinfection of needleless mechanical access ports: a matter of time and friction. Journal of the association of vascular access 12: 203-5

Reference 3:
Loveday HP, Wilson JA, Pratt RJ, Golsorkhi, A Bak JB, Prieto J and Wilcox M (2014) epic 3: National Evidence-Based Guidelines for Preventing Healthcare-Associated Infections in NHS Hospitals in England. Journal of Hospital Infection, supplement S1-S70

Reference 4:
Macklin D (2010) Catheter management. Seminars in Oncology Nursing. 26(2) 113-120

Reference 5:
NICE (2012) Infection: prevention and control of healthcare-associated infections in primary and community care. NICE, London

Reference 6:
O'Grady NP, Alexander M, Dellinger EP, Gerberding JL, Heard SO, Maki DG, Masur H, McCormick RD, Mermel LA, Pearson ML, Raad II, Randolph A, Weinstein RA (2011) CDC - Guidelines for the Prevention of Intravascular Catheter-Related Infections. Morbidity and Mortality Weekly Report 51: 1-26 

Reference 7:
Royal College of Nursing (2010) Standards for Infusion Therapy 3rd edition. RCN, London

Reference 8:
Rowley S, Clare S, Macqueen S, Molyneux R (2010) ANTT v2: An updated practice framework for aseptic technique. British Journal of Nursing 19(5)

Reference 9:
Rowley S, Clare S (2011) ANTT: a standard approach to aseptic technique. Nursing Times 107(36)

Reference 10:
Simmons S, Bryson C, Porter S (2011) “Scrub the hub” Cleaning duration and reduction in bacterial load on central venous catheters. Critical Care Nursing Quarterly 34(1): 31-35

Reference 11:
Smith JS, Irwin G, Viney M, Watkins L, Morris SP, Kirksey KM, Brown AB (2012) Optimal disinfection times for needleless intravenous connectors. Journal of the Association of Vascular Access 17(3): 137-43

Reference 12:
Soothill J, Bravery K, Ho A, Macqueen S, Collins, J, Lock P (2009) A fall in bloodstream infections followed a change to 2% chlorhexidine in 70% isopropanol for catheter connection antisepsis: a pediatric single centre before/after study on a hemopoietic stem cell transplant ward. American Journal of Infection Control 37(8): 626-30

Reference 13:
World Health Organisation (WHO) (2009) Guidelines on Hand Hygiene in Health Care: First Global Patient Safety Challenge, Clean Care is Safe Care. [Accessed 13 January 2015]

Document control information

Lead Author(s)

Anne Ho, Specialist Nurse, Intravenous Therapy

Additional Author(s)

Ellie Simmonds, Specialist Nurse, Intravenous Thearapy
Barbara Brekle, Deputy Lead Nurse Infection Control

Document owner(s)

Anne Ho, Specialist Nurse, Intravenous Therapy

Approved by

Guideline Approval Group

Reviewing and Versioning

First introduced: 
01 May 2003
Date approved: 
13 January 2015
Review schedule: 
Three years
Next review: 
01 August 2018
Document version: 
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